Plasmid_Backbone

Part:BBa_K5299006:Design

Designed by: Maria Nefeli Stoupa   Group: iGEM24_Thessaly   (2024-09-24)


Tn7-M: KmR GmR, ori R6K, Tn7L and Tn7R extremes, standard multiple cloning site


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 56
    Illegal XbaI site found at 83
    Illegal SpeI site found at 145
    Illegal PstI site found at 95
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 56
    Illegal SpeI site found at 145
    Illegal PstI site found at 95
    Illegal NotI site found at 46
    Illegal NotI site found at 113
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 56
    Illegal BglII site found at 411
    Illegal BglII site found at 2606
    Illegal BamHI site found at 77
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 56
    Illegal XbaI site found at 83
    Illegal SpeI site found at 145
    Illegal PstI site found at 95
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 56
    Illegal XbaI site found at 83
    Illegal SpeI site found at 145
    Illegal PstI site found at 95
    Illegal NgoMIV site found at 1645
    Illegal NgoMIV site found at 1649
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

R6K origin of replication requires a pir strain for replication


Source

Mini-Tn7 vectors have been developed and proven to be useful for integration of a cloned gene and genetic analysis of several Gram-

References